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Solutions and Reagents From sample preparation to detection, the reagents you dde for your Western 66 are now in one convenient kit: Treat cells by adding fresh media containing regulator for desired time. Blotting Membrane and Paper: Protein Blotting A general protocol for sample preparation. Aspirate media from cultures; wash cells with 1X PBS; aspirate. Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment.

The membrane is now ready to reuse.